Journal: Molecular Neurodegeneration
Article Title: Amyloid-β (Aβ) immunotherapy induced microhemorrhages are linked to vascular inflammation and cerebrovascular damage in a mouse model of Alzheimer’s disease
doi: 10.1186/s13024-024-00758-0
Figure Lengend Snippet: Increased fibrotic response around vascular amyloid deposits in 3D6 Treated PDAPP mice. a Double immunofluorescence of amyloid (X-34, blue) and fibroblasts (ERTR7, red) in PDAPP mice treated with 3D6 or IgG control. X-34 and ERTR7 immunoreactivity overlay (Merge). b Quantification of ERTR7 + area (%) in IgG or 3D6 treated PDAPP mice. Results are shown as mean ± SEM of n = 6 (mice). c Correlation of CD45 and Fibronectin from individual vessels. d Correlation of CD11b and Fibronectin from individual vessels. e Correlation of F480 and Fibronectin from individual vessels. Asterisks indicate significant differences, where * p < 0.05 and **** p < 0.0001 by unpaired Student’s t test. Scale bar 10 μm
Article Snippet: Sections were then incubated overnight at 4 °C with the following antibodies, each diluted 1:100 in blocking solution: anti-Fibrinogen ( PA1-85,429, ThermoFisher), anti-SMA (PA585070, ThermoFisher), anti-CD 31 (14–0311-82, ThermoFisher), anti-VEGF (19,003–1-AP, ThermoFisher), anti-Cldn5 (34–1600, ThermoFisher), anti-GFAP (13–030-0, ThermoFisher), anti-AQP4 (50–173-0968, ThermoFisher), anti-CD19 (14–0194-82, ThermoFisher), anti-CD3 (PIMA514524, Fisher), anti-CD4 (14–9766-82, ThermoFisher), anti-CD8 (14–0808-82, ThermoFisher), anti-Laminin (NB300-144,NovusBio) anti-MHC II (107,610, Citeab), anti-ERTR7 (NB100-64932, Novus) and anti-CD36 (18,836–1-AP, ThermoFisher).
Techniques: Immunofluorescence, Control